Professional Cell Culture
$1,500.00
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- Human Induced Pluripotent Stem Cell Techniques
Human Induced Pluripotent Stem Cell Techniques
- Professional Level: Industry scientists, postdocs, technicians and research associates
- Format/ Schedule: Spring / Winter
- Location Torrance, CA
- 2025 Dates: Inquire for 2025 dates
- Duration: 4 days
- Times: 10.00am-5.00pm, Monday-Thursday
- Class size: 8
Admission
Participants are accepted on a first-come-first-served basis up to 1 week before the course start date. Additional questions? Click here to learn more about our general admissions policy or call us at 310-483-3651.
Description
“hiPSC Techniques” provides comprehensive training in validated methods for deriving, culturing, characterizing, and differentiating human induced pluripotent stem cells (hiPSC).
Curriculum
Participants learn to:
– Reprogram hiPSC using Episomal plasmids and Sendi virus
– Select, prepare and aliquot hiPSC/hESC culture reagents
– Morphologically assess hiPSC/hESC colonies
– Culture hiPSC/hESC feeder free in mTeSR, NutriStem and StemFlex
– Pick and manually passage hiPSC/hESC colonies
– Passage hiPSC/hESC using Accutase, ReLeSR and Collagenase
– Cryopreserve and thaw hiPSC/hESC
– Prepare embryoid bodies (scraped and spin-EB’s)
– Differentiate hiPSC/hESC to specific germ layers
– Perform standard characterization for immunological markers, vector integration and pluripotency
– Quality control monitor hiPSC/hESC during prolonged culture
– Electroporate hiPSC/hESC
– Establish an hiPSC/hESC laboratory
– Troubleshoot common problems
– Comply with ESCRO regulations
– Reduce costs through optimized purchasing and laboratory organization
Our streamlined culture protocols have been validated for use with human induced pluripotent stem cells (hiPSC) and human embryonic stem cells (hESC). The course curriculum is updated annually to reflect current techniques.
Feeder-dependent culture methods can be taught upon request for an additional cost. Our scientists can also advise on approaches for deriving hiPSC from non-human species.
Features and Benefits
The program features:
– Experienced instructors with more than 20 years of cell culture experience
– An optimized and regularly updated curriculum that was originally developed in 2009 at the USC stem cell core facility
– Relevant learning experiences for scientists of all skill levels
– Streamlined procedures that will save you time and money
– Validated protocols and SOPs
– Continuing support with troubleshooting problems
– Networking with other science professionals
– Easy registration
– Affordable tuition
– Certificate of completion
We’ve helped hundreds of science professionals from around the world to gain new skills, enhance their resumes and advance in diverse careers!
Dr. Fox is a fantastic teacher
I signed up for the class with no real background in cell culturing or much of a microbiology laboratory background. After the class, I really enjoyed the techniques taught and would actually want to pursue a career more in line with a cell culturing discipline. It definietly gave me a step up in my resume as well as employers have definietly taken notice that I have a rudimentary background in cell culturing techniques and now I have a great job in a big biotech company and I just got out of college 8 months ago. Dr. Fox is a fantastic teacher who really explains the material in digestible chunks. The lab portions take up a good portion of your time to and should teach you all the essential techniques. Dr. Fox is always helpful and willing to ask as many questions as possible. Her lectures are also interesting and provide a great introduction into what lab work you’ll be doing that day. I’d recommend this to anyone who wants to boost their resume up and learn some additional skills that’ll be valued in the biological science job market.
I recommend this course to anyone
This was the most informative source of educational and hands-on training I have encountered in Human Pluripotent Stem Cell Techniques. The course coordinator, Dr. Victoria Fox is very knowledgeable, and her solid scientific research background in stem cells offered a good perspective of laboratory and clinical studies. The execution of the program was extremely well organized. It was small enough that the hands-on breakout sessions in the afternoon were intimate enough to fully participate and ask questions freely. This concise 5-day course changed my approach to stem cell acquisition and helped tremendously in my research. I recommend this course to anyone new or experienced in the changing world of stem cell research
A good teaching environment
This course is interesting, fun, and good training for working with not only human stem cells, but also teaches you good techniques for working with all types of cell culture. Dr. Fox gives students both guidance and freedom to attempt complicated assays within a good teaching environment.
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