Essential Cell Culture Techniques
$1,200.00
- Age: Any College Age
- Format/ Schedule: Spring / Fall
- Location Torrance, CA
- 2025 Dates: May 17th – 23rd | May 27th – Jun 2nd | Aug 23rd – 29th | Sept 2nd – 8th
- Duration: 7 Days
- Times: 10.00am – 5.00pm, Monday-Sunday
- Class size: 10
Eligibility
Advanced Stem Cell Techniques is an advanced hands-on program open to residential and international students of all levels – undergraduate and postgraduate. Applicants must have completed at least 1 year of an undergraduate degree in a bioscience related subject covering advanced cell biology. To attend this course, you must be proficient in aseptic technique and have prior experience culturing mammalian cells in a professional cell culture setting. Students who do not have cell culture experience can gain the required skills by attending our essential cell culture techniques program.
Admission
Eligible students are accepted on a first-come-first-served rolling basis up to 1 week before the course start date. Additional questions? Click here to learn more about our general admissions policy or call us at 310-483-3651.
Description
“Advanced Stem Cell Techniques” provides comprehensive training in validated methods for deriving, culturing, characterizing, and differentiating three types of human stem cells – neural (NSC), mesenchymal (MSC) and induced pluripotent (iPSC).
Curriculum
Participants learn to:
– Derive NSC from hiPSC
– Culture NSC as adherent monolayers and neurospheres
– Characterize and differentiate NSC
– Culture, characterize and differentiate MSC
– Perform cloning assays
– Reprogram hiPSC using Episomal plasmids and Sendi Virus
– Select, prepare and aliquot hiPSC/hESC culture reagents
– Morphologically assess hiPSC/hESC colonies
– Culture hiPSC/hESC under feeder free conditions in mTeSR and NutriStem
– Pick and manually passage hiPSC/hESC colonies
– Passage hiPSC/hESC using Accutase and ReLeSR
– Cryopreserve and thaw hiPSC/hESC
– Prepare embryoid bodies (scraped and spin-EB’s)
– Differentiate hiPSC/hESC to specific germ layers
– Electroporate hiPSC/hESC
– Establish an hiPSC/hESC laboratory
– Perform standard characterization for immunological markers, vector integration and pluripotency
– Quality control monitor hiPSC/hESC during prolonged culture
– Electroporate hiPSC/hESC
– Troubleshoot common problems
– Comply with ESCRO regulations
– Reduce costs through optimized purchasing and laboratory organization
Our streamlined hiPSC protocols have been validated for use with human embryonic stem cells (hESC). The course curriculum is updated annually to reflect current techniques.
Features
The program features:
– Highly sought after lab training
– Real-world lectures and technical information
– Professional hands-on experience
– Experienced instructors with more than 20 years of stem cell culture experience
– An optimized and regularly updated curriculum that was originally developed in 2009 at the USC stem cell core facility
– Relevant learning experiences for students of all skill levels
– Streamlined procedures that will save you time and money
– Validated protocols and SOPs
– Continuing support with troubleshooting problems
– Networking with other science professionals.
– Easy registration
– Affordable tuition
– Certificate of completion
– Career advise
We’ve prepared hundreds of students for advanced laboratory research and diverse careers.
Benefits
Students who take this course:
– Learn advanced skills that are highly sought by many bioscience employers
– Learn industry standard skills that are not taught by colleges or universities
– Gain comprehensive training in a professionally equipped biotech laboratory
– Avoid picking up “bad habits” through substandard training
– Expand their research options with stem cell models
– Reduce the burden on employers to provide specialized training
– Enhance their resume and expand their employment opportunities
Fantastic teacher
Dr. Fox is a fantastic teacher. She works closely with the students to be able to address shortfalls in their techniques. I appreciate the tips and practices that I learned in this course to improve my cell culture skills. I recommend this course to anyone looking to either address issues within their own technique or to advance their current knowledge with cell culture
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